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chemiluminescence reagents include caspase 3 activity detection kit  (Beyotime)


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    Beyotime chemiluminescence reagents include caspase 3 activity detection kit
    Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and <t>the</t> <t>caspase-3</t> activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.
    Chemiluminescence Reagents Include Caspase 3 Activity Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 4119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+activity+detection+reagent+kit/Caspase+3+Activity+Assay+Kit/pmc11091461-80-0-15
    Average 97 stars, based on 4119 article reviews
    chemiluminescence reagents include caspase 3 activity detection kit - by Bioz Stars, 2026-10
    97/100 stars

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    1) Product Images from "Translational selenium nanoparticles boost GPx1 activation to reverse HAdV-14 virus-induced oxidative damage"

    Article Title: Translational selenium nanoparticles boost GPx1 activation to reverse HAdV-14 virus-induced oxidative damage

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.04.034

    Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and the caspase-3 activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.
    Figure Legend Snippet: Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and the caspase-3 activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Techniques Used: Infection, Staining, Microscopy, Activity Assay, TUNEL Assay, Membrane, Labeling, Flow Cytometry, Fluorescence, Comparison, Virus

    SeNPs@LNT resists adenovirus pneumonia induced by HAdV-14 in mice. (A) Schematic showing the in vivo experimental design for the treatment of HAdV-14 infection; (B) Analysis of lung index in mice treated with SeNPs@LNT. After the mice in the virus group were infected with HAdV-14 through nose drops, the lung index was significantly higher than that in the control group; (C) Survival and body weight of mice in different groups at 14 d; (D) The lung tissue sections of mice in different treatment groups were stained with HE and the number of cells in each field was counted and analyzed by Image J. TUNEL test was performed on the lung tissue of mice to detect the DNA fragmentation; (E) Immunohistochemistry of the mice in different groups. The dark brown particles representing T-p38, T-p53, T-JNK, p -JNK (Thr183/Tyr185), T-Caspase-3, T-Akt, and T-Erk proteins expression in the lung tissue of the mice. The therapeutic concentration of SeNPs@LNT was 0.2 mg Se/kg. Bars with different characters are statistically different at P ＜0.05 (* or # ) or P ＜0.01 (**) level. * represents comparison with the Control group, # represented comparison with the Virus group.
    Figure Legend Snippet: SeNPs@LNT resists adenovirus pneumonia induced by HAdV-14 in mice. (A) Schematic showing the in vivo experimental design for the treatment of HAdV-14 infection; (B) Analysis of lung index in mice treated with SeNPs@LNT. After the mice in the virus group were infected with HAdV-14 through nose drops, the lung index was significantly higher than that in the control group; (C) Survival and body weight of mice in different groups at 14 d; (D) The lung tissue sections of mice in different treatment groups were stained with HE and the number of cells in each field was counted and analyzed by Image J. TUNEL test was performed on the lung tissue of mice to detect the DNA fragmentation; (E) Immunohistochemistry of the mice in different groups. The dark brown particles representing T-p38, T-p53, T-JNK, p -JNK (Thr183/Tyr185), T-Caspase-3, T-Akt, and T-Erk proteins expression in the lung tissue of the mice. The therapeutic concentration of SeNPs@LNT was 0.2 mg Se/kg. Bars with different characters are statistically different at P ＜0.05 (* or # ) or P ＜0.01 (**) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Techniques Used: In Vivo, Infection, Virus, Staining, TUNEL Assay, Immunohistochemistry, Expressing, Concentration Assay, Comparison

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    Activity Assay:

    Article Title: Silencing of the ABCC4 gene by RNA interference reverses multidrug resistance in human gastric cancer.
    Article Snippet: .. Cells from each treatment group were collected, and the activity of caspase-3, -8, and -9 was detected according to the instruction's manual included with the caspase activity detection reagent kit (Beyotime, China). ..



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    chemiluminescence reagents include caspase 3 activity detection kit  (Beyotime)
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    Beyotime chemiluminescence reagents include caspase 3 activity detection kit
    Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and <t>the</t> <t>caspase-3</t> activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.
    Chemiluminescence Reagents Include Caspase 3 Activity Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+activity+detection+reagent+kit/Caspase+3+Activity+Assay+Kit/pmc11091461-80-0-15
    Average 97 stars, based on 1 article reviews
    chemiluminescence reagents include caspase 3 activity detection kit - by Bioz Stars, 2026-10
    97/100 stars
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    caspase activity detection reagent kit  (Beyotime)
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    Beyotime caspase activity detection reagent kit
    Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and <t>the</t> <t>caspase-3</t> activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.
    Caspase Activity Detection Reagent Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+activity+detection+reagent+kit/Caspase+6+Activity+Assay+Kit/pm25572969-66-25-30
    Average 94 stars, based on 1 article reviews
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    Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and the caspase-3 activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Journal: Bioactive Materials

    Article Title: Translational selenium nanoparticles boost GPx1 activation to reverse HAdV-14 virus-induced oxidative damage

    doi: 10.1016/j.bioactmat.2024.04.034

    Figure Lengend Snippet: Intracellular localization of HAdV-14 and the inhibitory effect of SeNPs@LNT on apoptosis induced by HAdV-14 infection. (A) DAPI was used for nuclear staining and Lyso-tracker was used for lysosome staining. The A549 cells were infected by rAD14 EGFP for different periods and were observed under the fluorescent microscope; (B) Schematic diagram of the infection process of HAdV-14 in A549 cells; (C) HAdV-14 infection of A549 cells were treated with SeNPs@LNT and the caspase-3 activity was detected by the absorbance of pNA; (D) DNA fragmentation and nuclear deformation in A549 cells were detected by TUNEL-DAPI co-staining assay; (E and F) A549 cells in each group were stained with Annexin V-FITC and PI to detect phosphatidylserine on the cell membrane surface. The results of fluorescent labeling were observed by flow cytometry and fluorescence microscopy. The A549 cells were treated with 8 μM SeNPs@LNT for 48 h. Bars with different characters are statistically different at P ＜0.05 (* or # ) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Article Snippet: Chemiluminescence reagents include Caspase-3 activity detection kit and enhanced cell counting kit-8 (CCK-8) purchased from Beyotime (China).

    Techniques: Infection, Staining, Microscopy, Activity Assay, TUNEL Assay, Membrane, Labeling, Flow Cytometry, Fluorescence, Comparison, Virus

    SeNPs@LNT resists adenovirus pneumonia induced by HAdV-14 in mice. (A) Schematic showing the in vivo experimental design for the treatment of HAdV-14 infection; (B) Analysis of lung index in mice treated with SeNPs@LNT. After the mice in the virus group were infected with HAdV-14 through nose drops, the lung index was significantly higher than that in the control group; (C) Survival and body weight of mice in different groups at 14 d; (D) The lung tissue sections of mice in different treatment groups were stained with HE and the number of cells in each field was counted and analyzed by Image J. TUNEL test was performed on the lung tissue of mice to detect the DNA fragmentation; (E) Immunohistochemistry of the mice in different groups. The dark brown particles representing T-p38, T-p53, T-JNK, p -JNK (Thr183/Tyr185), T-Caspase-3, T-Akt, and T-Erk proteins expression in the lung tissue of the mice. The therapeutic concentration of SeNPs@LNT was 0.2 mg Se/kg. Bars with different characters are statistically different at P ＜0.05 (* or # ) or P ＜0.01 (**) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Journal: Bioactive Materials

    Article Title: Translational selenium nanoparticles boost GPx1 activation to reverse HAdV-14 virus-induced oxidative damage

    doi: 10.1016/j.bioactmat.2024.04.034

    Figure Lengend Snippet: SeNPs@LNT resists adenovirus pneumonia induced by HAdV-14 in mice. (A) Schematic showing the in vivo experimental design for the treatment of HAdV-14 infection; (B) Analysis of lung index in mice treated with SeNPs@LNT. After the mice in the virus group were infected with HAdV-14 through nose drops, the lung index was significantly higher than that in the control group; (C) Survival and body weight of mice in different groups at 14 d; (D) The lung tissue sections of mice in different treatment groups were stained with HE and the number of cells in each field was counted and analyzed by Image J. TUNEL test was performed on the lung tissue of mice to detect the DNA fragmentation; (E) Immunohistochemistry of the mice in different groups. The dark brown particles representing T-p38, T-p53, T-JNK, p -JNK (Thr183/Tyr185), T-Caspase-3, T-Akt, and T-Erk proteins expression in the lung tissue of the mice. The therapeutic concentration of SeNPs@LNT was 0.2 mg Se/kg. Bars with different characters are statistically different at P ＜0.05 (* or # ) or P ＜0.01 (**) level. * represents comparison with the Control group, # represented comparison with the Virus group.

    Article Snippet: Chemiluminescence reagents include Caspase-3 activity detection kit and enhanced cell counting kit-8 (CCK-8) purchased from Beyotime (China).

    Techniques: In Vivo, Infection, Virus, Staining, TUNEL Assay, Immunohistochemistry, Expressing, Concentration Assay, Comparison